Association of MMP-2 (-1306 C>T), MMP-9 (-1562 C>T) Gene Polymorphism and the Formation of the Hematological Malignancies

Matrix metalloproteinases (MMPs) are a large family of zinc-containing proteases several functions of the MMPs were identified in various systems such as cell migration/invasion, apopto-sis/cell survival, and angiogenesis (1). Besides, MMP-2 and MMP-9 have important implications in the progression and invasiveness of several hematological malignancies, including Hodgkin lymphomas (HL), malignant non-Hodgkin lym-phomas (NHL), chronic myeloid leukemia (CML) and acute myeloid leukemia (AML) (2). Functional single nucleotide polymorphisms (SNPs) have been identified for the two molecules: SNP in the promoter of the MMP-2 gene (-1306 C>T) 1 and MMP-9 gene (-1562 C>T) polymorphisms (3) that have effect the expressions of the genes. The aim of the study was to assess the relationship between the SNPs and hematological malignan-cies. This cross-sectional and retrospective analysis was performed for the patients diagnosed between 2006-2007 at Mersin University Hospital. The patient group was comprised of 52 chronic lymphocytic leukemia (CLL), 20 HL, 38 NHL, 15 acute lymphocytic leukemia (ALL), 14 multiple myeloma (MM) patients. The control group included 110 healthy individuals. The study was approved by the local Ethic Committee and informed consent was obtained from all patients. DNA was extracted from venous blood and used for the polymerase chain reaction, restriction fragment length polymorphism (PCR-RFLP) assays. MMP-2 genotypes were CC, CT and TT and had band sizes of 193-bp, 193/167/26-bp, 167/26-bp PCR product and MMP-9 genotypes were CC, CT and TT and had band sizes of 436-bp, 436/242/194-bp, 242/194-bp PCR product, respectively for the SNPs.